The primers used to clone the 1.5 Kb DNA fragment before miR-2b-1 using the same method were: forward, 5′-GCGTGCTAGCCCGGGCTCGAGTTTAAATGTGCTTTTTTAAATAGCGAGCCACTG-3′; reverse, 5′-AAGCTTACTTAGATCGCAGATTGAATATTGTTGACAACATGTCACTGCCAC-3′.
The third approach, the income method, is forward-looking, relying on the present value of expected cash flow.
One such method is forwarding the messages through the friends of source node or destination node.
In conclusion, a qPCR method is forwarded here that could be useful as an early warning tool for fast and reliable detection of Microthrix in for instance sludge bulking events.
Two widely used search methods are forward selection (FS) and backward elimination (BE) [ 15].
Aimed at the optimized lighting quantity and quality, an improved designing method was put forward.
Additionally, a summation and comparison combination method was put forward and different defect signal amplitudes were compensated.
Therefore, a specific micro-fracture plugging evaluation method was put forward especially for high-temperature deep wells in the paper.
For the purpose of precisely calculating the volume of cylindrical spur gear billet in cold precision forging, a new theoretical method named average circle method was put forward.
The DEA method was first put forward by some famous operational research experts.
