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The distribution derived in this study can be used as a bench mark against which to evaluate LOD50 data generated in future studies of detection methods for Salmonella in foods and potentially offers a possible way to streamline method validation study experimental design.
Method validation study was performed based on the current pharmaceutical regulatory guidelines i.e., ICH Q2 (R1).
The contributions of repeatability to the measurement uncertainty were combined into one contribution for the overall experiment and were obtained from the method validation study performed in the laboratory.
A method validation study showed that the LC/MS technique was a powerful analytical tool for detecting trace amounts of the flavonoid compounds in extracts of S. involucrata (Xu et al. [2009]).
Three of the DCM patients were included in a prior method validation study.
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Method validation studies showed that the proposed method can be used to prepare trans-resveratrol.
Method validation studies also evidenced that ILSMP-UMSE is credible for the preparation of two coumarins from Cortex fraxini.
Although this hierarchy forms the basis of most exposure assessment method validation studies [ 31, 32], it is rare to find validation studies that quantitatively demonstrate a difference in precision between methods rather than just agreement.
Method validation studies showed that average recoveries ranged from 82.8% (diclofenac) to 100.6% (carbamazepine) from the soils and from 86.3% (fluoxetine) to 100.9% (carbamazepine and diclofenac) from the earthworms.
Although, the subject of method validation studies in the past, these have tended to focus on either precision of quality control samples or between laboratory concordance (reproducibility) [ 15, 16].
To assess changes in the levels of metabolites throughout the timecourse, relative metabolite levels at each timepoint were compared to the 24 h timepoint, which was the incubation time used in the method validation studies.
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