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We first report multiple skipping of dystrophin exons 6 and 8 in the DMD patient's cells and translation of the unified antisense PMO design from a DMD dog model to a human based on the MyoD-transduction method utilizing flow cytometry.
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Label-free methods utilize flow parameters of channels of various shapes and sizes to manipulate streamlines carrying cells and particles.
Here we describe a new method utilizing multicolor flow cytometry to isolate pure populations of EC from the skeletal muscle based on the antigens Sca-1, CD34, CD31 and CD45.
We then used these markers to develop a method utilizing multicolor flow cytometry to exclusively sort muscle derived venular, arterial and capillary EC which are all positive for Sca-1, CD31 and CD34 while excluding hematopoietic cells which express the common leukocyte antigen CD45.
The method utilizes open-flow respirometry, a technique that allows for the measurement of cellular respiration at steady state (e.g. O2 supply = O2 demand).
Nampt/PBEF/visfatin in sera and tissue homogenates was measured by means of flow cytometry-based method utilizing magnetic microspheres conjugated with monoclonal antibodies using the BioPlex 200 platform with HRF (Bio-Rad, USA), incorporating Luminex xMAP technology, and Bio-Plex Pro Human Diabetes Assay Panels.
We have developed an electrochemical fabrication method utilizing breakdown anodization (BDA) to yield capillary flows that can be expressed as functions of capillary height.
This paper applies a new method utilizing shuffled frog-leaping algorithm and three phase probabilistic load flow combined with the Monte Carlo method to solve this problem.
Cell volume was determined utilizing flow cytometry.
The method utilizes process data from flow sheet diagram, which is already available at the preliminary design stage.
This versatile method utilizes stress-induced atomic mass flow along grain boundaries in the polycrystalline film to form nanowires.
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