Sentence examples for method using wild from inspiring English sources

Based on the bioassay method using wild type inbred mouse lines, BSE was shown to be caused by a single strain of agent that produced a unique lesion profile and incubation period (17).

Our data therefore reveal a broad conservation of replicative helicase loading mechanisms across kingdoms, which can hopefully be further elucidated by crystallographic or other biophysical methods using wild type and mutant helicase loading complexes.

A mismatched PCR method, using one wild-type primer and one containing the SNP which matched/mismatched the template DNA at the 3'-end of the primer (Table 2), was used to detect SNPs in the Beijing isolates from China (CN).

Copy number was calculated with the ΔΔ C t (C t is threshold cycle value) method using a wild-type male with one Plp1 gene as the calibrator (Regis et al., 2005).

In the present study, an approach for using Wild Cards in research was developed using different methods and perspectives by combining Wild Cards with a classical SWOT analysis and ongoing trends.

Ear tagging is a common and safe method used for wild mice.

Although the overall heritable mutation rates are high (12.4 74.2%), the germ-cell specificity of genomic editing was not examined, and this system is available only for Cas9-expressing transgenic lines, in contrast to our method, which uses wild-type animals.

Attenuated reovirus derived from the HTR1 culture was purified by the same method used in wild-type reovirus preparation, except AV reovirus was propagated in HT1080 and L929 cells.

Percent of wild-type expression was calculated using the ΔΔCT method using ef1a as the standard and wild type as the reference.

The genome copy number of the Tg mice was estimated by the comparative Ct method using the amplification curve of the wild genome as a standard [51].

The crystals were then soaked with 75 mM Neu5Ac, and the three-dimensional structure was solved (PDB code 4BWL; see Supplementary Table S1 for crystallographic data) by direct Fourier methods using the wild-type E. coli structure as a starting model.