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The flsQ1 gene was inactivated by PCR-targeting method using the apramycin resistance cassette8.
The lattice parameters were obtained by the whole-pattern profile matching method using the FULLPROF52 program.
A distance matrix that was clustered using the "average" linkage method (using the R hclust function).
Computer scientists had previously relied on a method using the simplex algorithm to review and order vast stores of information.
Adsorption isotherms for CH4 and N2 at all temperatures were computed by the GCMC method using the RASPA software35,36.
SAPAP1 expression was measured by the RT-PCR method using the ABI PRISM 7700 (Perkin-Elmer) as described.
We validate our analytical method using the ns-2 simulator.
An efficient and site-specific F18-labeling method using the rapid condensation reaction between 2-cyanobenzothiazole and cysteine.
We demonstrate our method using the PAM50 classifier and its associated University of North Carolina (UNC) training cohort.
The relative expression values of each transcript were then calculated by the delta-delta Ct (2−ΔΔCt) method using the CFX Manager 3.0 of the amplifier.
The relative mRNA levels were calculated using the comparative Ct method, using the house keeping genes β-actin and GAPDH as an internal control.
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