Sentence examples for method using purified from inspiring English sources

An ELISA method using purified tyvelose-containing antigen from muscle larvae of T. spiralis is sensitive and specific for immunodiagnosis of trichinellosis, but it is not useful for early diagnosis [ 9].

TST was conducted by Mantoux method using purified protein derivative (PPD, Statens Serum Institute; Copenhagen, Denmark).

Tuberculin skin tests were performed at baseline and one year follow-up by the Mantoux method using purified protein derivative (5 tuberculin units of Tubersol PPD, Connaught, Swiftwater, Pennsylvania, USA) [ 19].

The library was prepared by high-salt reconstitution methods using purified histones from chicken erythrocytes and naked high molecular weight yeast DNA using a DNA: histone mass ratio of 2.5∶1.

At the end of this project it was concluded that enzyme-linked immunosorbent assay (ELISA) methods using purified native PR3 and MPO, directly coated onto microwells, had been standardized, and it was confirmed that these methods were useful in differentiating patients with recent onset SVV from those with other systemic inflammatory diseases.

Validation of the method by using purified enzyme with a known concentration showed that the method is accurate.

This method was also repeated using purified ligation mixtures and the results were comparable (data not shown).

All crystallization experiments were conducted using vapor diffusion methods and performed as follows using purified N-labeled S100B (bovine) protein.

The selected BSs were confirmed by at least one of the following methods: (1) DNase I footprinting using purified protein, (2) electrophoretic mobility shift assay (EMSA) with purified protein, and (3) EMSA with nuclear extract and specific antibodies.

In this method, we used purified recombinant 120 kDa DPP8 or DPP9 protein from the Rosetta expression system.

A second method for calibration used purified protein standards in the following way: 5 µL of purified GFP solution containing ∼1012 molecules of GFP were placed under a slide cover slip with diameter of 13 mm.