Sentence examples for method using polyclonal from inspiring English sources
We have developed an alternative method using polyclonal antibodies immobilized on ProteinChip Arrays and SELDI-TOF mass spectrometry.
We examined the intracellular location of tRNase ZL by the indirect fluorescent method using polyclonal antibodies against a human tRNase ZL peptide (C-terminal amino acid 812 826).
Immunostaining was carried out on paraffin-embedded tissue sections by streptavidin biotin peroxidase complex method using polyclonal antibodies for KLK6 and KLK10 (1 : 150).
Immunohistochemistry was performed with the EnVision method using polyclonal rabbit anti-horse antibodies specific for AA amyloid (Anti amyloid K-10a) in 1/100 dilution with phosphate buffered saline and bovine serum albumin.
Western blot analysis was performed by the chemiluminescence method, using polyclonal antibodies against a peptide from AtMHX sequence (Cys-Glu-Glu-Ile-Asp-Thr-Ser-Lys-Asp-Asp-Asn-Asp-Asn-Asp-Val-His-Asp) that were affinity-purified prior to use against the same peptide using the SulfoLink Coupling Gel (Pierce).
This method uses polyclonal antibodies to estradiol (E2) to immunoprecipitate these major biologically active steroidal estrogens, leaving behind the nonsteroidal estrogens, which are then detected in a cell-based transcriptional activation bioassay for estrogen receptor agonist.
Immunoblotting was done using standard methods using polyclonal mouse anti-CTD (8WG16).
SDS-PAGE and immunoblotting were performed by standard methods, using polyclonal antibodies 1∶10 000 in 1% v/v skimmed milk in TBS-T (0.05% v/v Tween-20, 25 mM Tris-HCl, 150 mM NaCl, pH 7.5), followed by 1∶5 000 horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (Dako).
We also used both wild-type and white eye mutants for electron microscopy of preparations immunolabeled for glutamate by the pre-embedding method using the polyclonal rabbit anti-glutamate [62].
Serial frozen sections were used to localise VEGF by indirect immunoflourescence method using a polyclonal antiserum made against recombinant human VEGF165 (Olson et al, 1996).
We chose to use a well-established method using a polyclonal antibody against hTERT to immuno-purify telomerase from these cell lines (Cohen & Reddel, 2008).
