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The iron oxide nanoparticles were synthesized by the preferred thermal decomposition method using organic solvents.
The iron oxide nanoparticles were synthesized by thermal decomposition method using organic precursors [40, 41].
Protein precipitation method using organic solvent is the simplest one but the chances of matrix effect prevail.
Therefore, SCW extraction is an alternative green technology for phenolic compounds extraction from agricultural wastes, which substitute conventional method using organic solvents.
The purities of crude peptides cleaved from the resin were over 90% and similar to the results by the general Fmoc synthesis method using organic solvents.
Since EBN is produced from swiftlet's saliva, extraction method using organic solvent such as methanol, chloroform and DMSO, which target the non-water soluble active compound, may not be practical.
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Wang and Weller (2006) described supercritical method as a significant substitute to conventional extraction methods using organic solvents for extracting biologically active compounds.
In addition to its low toxicity and environmental impact, supercritical CO2 extraction replaces conventional extraction methods using organic solvents that require numerous purification processes to remove chemical contaminants.
The most used carotenoid and chlorophyll extraction methods use organic solvents.
However, these chemical methods use organic solvents and toxic reducing agents, consume high energy, and require difficult waste treatment.
The DPPH method use organic radicals [ 19] and the FRAP assay can react with iron (II) and SH-group containing antioxidants [ 15], so it is expected that using these two methods accurately reflects all of the antioxidants in a sample.
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