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The azelaic acid content in tobacco leaves was determined by the modified GC-MS method using methyl derivatives and by solid-phase microextraction (25).
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Methods based on analysis of 5′ ends of cDNAs either involve low throughput methods eg using methyl cap [ 32] or have amplification biases that are less reliable in strand specificity (methods using SMART cDNA amplification) [ 33, 34].
Vertically aligned carbon nanotubes on a silicon substrate were synthesized by a spray pyrolysis method using the methyl ester of Helianthus annuus oil as a carbon source, ferrocene as a catalyst precursor and argon as a carrier gas.
Total alkalinity (as HCO3) was determined by acid titration method (0.1 N HCl) using methyl orange as an indicator.
Activity was estimated by using methyl rhodanine method.
The synthesized nanocomposites were chemically deposited on low carbon steel specimens by solvent evaporation method using N-Methyl-2-pyrrolidone (NMP) as solvent and 10% epoxy resin (by weight) as binder.
We have developed an approach that has been adapted from a method that uses methyl sensitive restriction enzymes.
Nuclei were counterstained using methyl green (Vector Laboratories).
ALP activity was measured by a kinetic rate method using a 2-amino-2-methyl-1-propanol (AMP) buffer (within run precision 1.8 and between run 2.1 at 150 iu/L).
In order to verify the modulation of autophagy using the typical induction method by starvation and the inhibition method using the pharmacological inhibitor 3-Methyl Adenine (3MA) in the human PBMCs system used in this study, Western blotting of the two LC3 fractions was performed.
Poly(GMA-co-MMA) (I), poly(GMA-co-EMA) (II), poly(GMA-co-MA) (III) and poly(GMA-co-BA) (IV) were synthesized by a general method using 2 g (20 mmol) of methyl methacrylate, 2 g (20 mmol) of ethyl methacrylate, 1.73 g (20 mmol) of methyl acrylate or 2.56 g (20 mmol) of butyl acrylate and also a mixture of 2 g (20 mmol) of GMA and 0.065 g (0.4 mmol) of AIBN in 15 mL of tetrahydrofuran (THF).
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