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The blood samples were processed according to a density gradient centrifugation method using Lymphocyte Separation Medium (MP Biomedicals Japan, Tokyo, Japan) to obtain a leukocyte-enriched white buffy coat.
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Bone marrow mononuclear cells (BM-MNCs) were isolated from fresh, unsorted bone marrow aspirate specimens (N = 69) by the Ficoll method using Cellgro Lymphocyte Separation Medium (Corning, Manassas, VA).
HLA-B type was determined by SRL Inc. (Tokyo, Japan) using lymphocyte cytotoxicity assay or a PCR reverse sequence specific oligonucleotides method.
To identify loci associated with lymphocyte counts, we conducted a GWAS using lymphocyte counts from 462 Hutterite subjects as the phenotypes, and genome-wide genotypes (using the Affymetrix 500k array platform; see Materials and Methods; 24, 26, 27).
Previously, HLA typing was done by two methods: serologic method using antiserum and mixed lymphocyte culture (MLC).
Lymphocyte cell viability was assessed by the ATP staining method using the CellTiter-Glo™ Luminescent Cell Viability Assay Kit.
For instance, Rasmusson and coworkers demonstrated that MSCs-mediated suppression is dependent on the method used to stimulate lymphocytes [ 53].
Human monoclonal antibodies (HuMAbs) were generated with hybridoma fusion methods using regional nodal lymphocytes of colon carcinoma patients.
Another method uses mats.
Most EPC quantification methods using flow cytometry identify cells within the lymphocyte gate or involve a lymphocyte separation step.
To optimize lymphocyte recovery, we compared two isolation methods using brains harvested from seven-day Theiler's murine encephalomyelitis virus (TMEV) and TMEV-OVA infected mice.
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