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The suspension of nanoparticles were dried at room temperature (method A), by slow freeze method in freeze at −20 °C (method B) and by fast freeze method using liquid nitrogen (method C) to form the solid nanoparticles.
Luque-Pérez et al. [9] reported a method using liquid membranes, in which the samples are directly placed in the membrane unit and nicotine released from the samples passes through the membrane (polypropylene/n-undecane) into an acidic acceptor stream.
The presented method employs liquid chromatography tandem mass spectrometry (LC MS/MS) as an alternative to a previously validated method using liquid chromatography-time-of-flight mass spectrometry (LC-TOF/MS).
Although the transformation frequency in liquid IM was 100-fold lower than that on solid IM, the AMT method using liquid IM is better suited for high-throughput insertional mutagenesis because the transformants can be isolated on fewer selection media plates by concentrating the transformed germlings.
Quantification of artemether and dihydroartemisinin was performed by a previously published method using liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) (45).
Therefore, sponges were first fixed with paraformaldehyd then dehydrated in acetone and finally dried with the critical point method, using liquid CO2 as exchanging medium (CPD 030, Bal-Tec AG, Liechtenstein).
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The distribution coefficients (logD values) of the radioligands were determined by a shake-flask method using liquid-liquid extraction followed by phase separation, as previously reported (Additional file 1) [31].
This method uses liquid urea, a chemical compound commonly synthesized for a variety of industrial uses, which is injected into the exhaust to effectively break down the pollutant.
This method uses liquid nitrogen-cell breakage to leach cytosol from cells prior to fixation.
Briefly, the method uses liquid chromatography separation with detection by tandem mass spectrometry.
Considering this restriction, AWD assays with bacteria inoculated onto agar plates could present some advantages in comparison to methods using liquid broth.
More suggestions(15)
methods using liquid
method using separate
method using low
method using multiple
method using recombinant
method using residual
method using nonmechanical
method using electric
method using standard
method using bovine
method using various
method using centrifuge
method involving liquid
method using sulfuric
method employing liquid
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