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Cell attachment, spreading and proliferation on the glass are determined by MTT [3- 4,5-Dimethylthiazol-2-yl -2,5-Diphenyltetrazolium Bromide] assay method using Human osteosarcoma MG-63 cells.
In order to control the power efficiently and effectively, energy saving method using human activity patterns should be considered.
The proposed system is applied as a potential energy saving method using human activity pattern and real-time positioning data.
The acetylcholinesterase inhibition effect of the isolated compounds (1 4) was assessed by Ellman's spectrophotometric method using human recombinant AChE with tacrine as reference compound.
Most positive results in MBP-ELISA correlated with positive anti-Sa results on the original WB method using human placenta.
The Gα chimeras (except C128) were constructed as described previously [ 36] by PCR method using human Gα16 and Gαz cDNAs.
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This evaluation method uses human observers to judge the behaviour shown in various video clips, then uses these judgements in correlation with those of the software.
Depending on the mosquito collection method used (human landing, light trap, etc)., the human-biting rate can be correctly approximated either by the number of blood meals taken on humans/mosquito/day or by the mosquito density.
Allogenic material is either obtained from cadaveric dermis or fascia, or engineered by methods using human cell lines and has a high biocompatibility with low antigenicity.
Although this method gave rise to BMS containing adipocytes, their number was considerably lower than that achieved with established methods using human BM aspirates in long-term culture (Coutinho et al, 1993).
The most commonly used DNA quantification methods, such as ultraviolet spectrophotometric absorbance ("UV") and fluorescent dyes such as PicoGreen® ("PG") do not differentiate between human and non-human DNA, but quantitative real time Polymerase Chain Reaction ("qPCR") or hybridization methods using human specific oligonucleotide primers are human DNA specific [ 10- 13].
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