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The method uses specific mapping functions in a way that minimizes the error dynamics close to zero.
This method uses specific primers designed to amplify a small genomic fragment of CAV-2 structural genes (pVI-hexon).
This method uses specific morphological characteristics to differentiate parasites.
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In addition, we tested the method using specific concepts on the Family and PokerHand datasets.
Although bioinformatics (http://rice.plantbiology.msu.edu) shows that there is another transcript, we haven't detected it by RT-PCR method using specific primers (data not shown).
Other methods are based on the comparison of the collected signal with an expected light shape [23], on the adoption of a center of gravity method, using specific algorithms to correct the artifacts at the edges of the scintillator [24], or on the optical coupling of neighbor crystals [25, 26].
MfdD778A having point mutation in Walker B motif of the ATPase domain was generated by mega primer method, using specific primer set WbF-WbM whereas WbF-WbR primer set was used for screening and confirmation of mutation.
The analytical procedure was based on a Real-Time PCR method, using specific primers for the amplification of the CYP21A2 gene, and TaqMan® (Applied Biosystems) probes as previously described [37].
For example, Cohen (2008) explores a hotspot identification method using specific words of interest.
P1 and P6 regions of Gata3 and α-SMA[ 41] promoter gene were amplified from mouse genomic DNA by RT-PCR method using specific primers.
For miRNA detection, we employed Poly (A) RT-PCR method using specific forward primers and a universal reverse primer complementary to an anchor primer as previously described [ 31].
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