Two series of hyperbranched polymers (HP), polyurethanes and polyureas, with aromatic and aliphatic structures, are synthesized in one-pot method using commercially available monomers.
Serum IgA levels were determined by nephelometric method, using commercially available kit (Siemens Healthcare Diagnostic Products Gmbh, Germany).
Fasting insulin will be assayed by (Radioimmunoassay) RIA method using commercially available insulin RIA kit supplied by Diagnostic Systems Laboratories Inc., Texas.
Effect of kinase inhibitors on cell proliferation was measured using BrdU incorporation method using commercially available kits (Roche Diagnostics, Indianapolis, IN, USA).
The sandwich ELISA (enzyme-linked immunosorbent assay) method using commercially available tests (Quantikine ELISA Kit, R&D Systems, Minneapolis, USA) was applied.
Minimum inhibitory concentrations (MIC) of ciprofloxacin and ceftriaxone were determined by the E-test method, using commercially available strips (AB Biodisk, Sweden), as per manufacturer's specifications.
The MMP-8 and -9 GCF concentrations were evaluated with the ELISA method using commercially available kits (R&D Systems, Minneapolis, MN, USA) according to the producer's manual.
Activities of the two liver enzymes aspartate transaminase (AST) and alanine transaminase (ALT in the plasma were measured by spectrophotometric method using commercially available assay kits (DiaSys Diagnostic Systems GmbH & Co. KG, Germany).
Glucose, TG, non-esterified fatty acid (NEFA), total cholesterol, and HDL cholesterol concentrations were determined by enzymatic colorimetric methods using commercially available kits (Roche Diagnostics Gmbh, Mannheim, Germany; Wako Chemicals GmbH, Neuss, Germany and Randox Laboratories Ltd., Co. Antrim, Ireland).
