Sentence examples for method used did from inspiring English sources

For control purposes, CHO-R1.1 cells transduced with a retroviral vector encoding cytoplasmic EGFP from pLEGFP.C1 (Clontech) were also used to confirm that the biotinyation method used did not label cytoplasmic proteins (data not shown).

The method used did not allow for dihydrouridine (D) and queosine (Q) derivatives to be analyzed.

The localisation method used did not involve the definition of 3D tumour volumes and is demonstrated by Fig. 1.

For the association detected between T, T1* and T2* subclades, the haplogroup inference method used did not bias the counts of affected and unaffected individuals in these subclades.

As no cost-benefit, cost-opportunity or cost-effectiveness analyses were carried out, the method used did not address economic evaluation of possible research returns.

The analysis also shows that low copy SoPIP1 2 clones were recovered at high antibiotic concentrations indicating that the zeocin selection method used did not completely exclude the occurrence of so called false positives (see discussion).

First, the sampling method used does not permit us to know how representative our samples were of the earthquake victim population.

Although the cytoplasmic staining may represent newly synthesized CD109 protein and/or CD109 protein that has undergone internalization, the method used does not allow us to precisely define the localization of CD109 in these cells.

This memory saving can be further enhanced if the method used does not require assembly of the full coefficient matrix during the solution procedure.

Also, the method used does not allow for any assumptions on the location of the Twitter user since the time series of tweets were collected based on user and not location.

Note that the extraction method used does not extract chromatin-bound proteins.