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The original method used a modified mouse leukemia virus to insert genes.
The second method used a desiccant, a sponge-like hydrating agent that attracts moisture from the atmosphere.
But, Dr. Wiles said, Dr. Flach's method, "used a lot of sophisticated machinery, and I didn't feel completely comfortable with it". As months went by, Dr. Wiles finally decided that he needed help.
The method used a novolac epoxy resin in combination with an amine curing agent (without ultra-violet curing).
Unlike other conventional methods employing various force sensors, this method used a Hall-current-sensor to dynamically measure the operating current of a permanent magnet dc-motor.
The method used a reversed-phase C18 column with a mobile phase composed of trifluoroacetic acid solution (0.1%, v/v) and acetonitrile in a gradient mode.
A second method used a commercially available cryomicroscopic setup to visualise the cells in suspension which involves a LINHAM cryostage FDCS 196.
A 'continuum of complexity' of long-term end-user involvement in the project is described, and the stakeholder involvement method used – a co-design process – is outlined.
The method used a sample of 484 agreements for which data were collected from surveys, a desk study and an interview with the agreement holder.
The modified SOBI method used a windowing technique to maximize the amount of information used for blind identification from the responses.
Independently of the minimization method used, a threshold value should be used as a stopping criteria for the iterative minimization procedure.
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