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The pulse-feeding assay, by measuring de novo protein synthesis, represents an ideal method to unequivocally establish how the composition of food dictates protein synthesis.
Similar to acetylation and phosphorylation, site-specific detection of modified residues in target proteins would be the most rigorous method to unequivocally identify modified proteins, but this is still challenging.
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At present, we lack methods to unequivocally determine the predominant mechanism of reduced insulin production at different stages of disease.
All this is further complicated by the lack of a specific marker and validated methods to unequivocally identify this circulating cell subset and by the presence of platelet microparticles in a standard assay for putative EPCs (41).
Cultivation methods failed to unequivocally identify a specific bacterial pathogen or unique pathogenic community in subjects with BV.
Nevertheless, our approach based on PCR and dot-blot hybridization methods is not sufficient to unequivocally consider that repertoires of T3Es determine host specificity in Xanthomonas axonopodis pathovars.
These results reveal that the developed method is a rapid and efficient tool to unequivocally identify S. scombrus and may aid in the prevention of fraud or mislabelling in mackerel products.
Based on this pilot study, sensitivity and specificity values of the system examined on a test database were not found high enough to unequivocally support its use as a pre-screening method prior to currently applied screening procedures.
Gaventa and McGee (2013) note that "[a] number of good, specific studies exist, using a range of methods, but there are [currently] not enough of these, across enough settings and methods, to begin to point unequivocally to overall patterns or to draw higher-order conclusions" [ 13].
However, to unequivocally prove the presence of phosphoarginine in histone proteins, direct phosphoarginine detection methods such as recently optimized mass spectrometry techniques or P NMR analysis should be employed.
The world must stand together to unequivocally reject these brutal acts".
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