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Molecular self-assembly of organosilanes has been proven to be a powerful method to rationally engineer the ceramic oxide surface properties [26 29].
We have developed a structure-based method to rationally predict single mutations at protein protein interfaces that enhance binding affinities.
The method proposed in this paper was proven effective, and we recommend it as an alternative method to rationally find a suitable temperature for protein crystallization screens.
In summary, we have demonstrated an efficient and easy to implement method to rationally predict engineering strategies for the improvement of production hosts.
Ming and Wall proposed a method to rationally optimize the parameters used in a CG-EN model by minimizing the Kullback-Leibler divergence (KLD) between the coarse-grained Hessian (from ANM) and the atomistic Hessian (from NMA) (Ming and Wall, 2005).
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Different transformation techniques as well as DNA- and RNA-based methods to rationally design metabolic fluxes have been developed for industrially important filamentous fungi.
The most widely accepted method to deal rationally with uncertainties is the stochastic approach including Bayes and Laplace's subjective interpretation of probability as a state of information.
Visualization of binding site hot spots makes this method suitable to rationally optimize the selectivity profile of a compound.
In this article, with the application of selection criteria for both Leishmania major targets (e.g. in silico gene lethality) and drugs (e.g. toxicity), a method (MetDP) to rationally focus on a subset of low-toxic Food and Drug Administration (FDA -approved drugs is introduced.
Our framework provides a way to critically evaluate the performance of segmentation methods, and therefore to rationally select one or several of them for a particular data set.
Here, we present a method to generate, evaluate, and optimize rationally designed endonucleases that promote HR.
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