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Kong et al. [ 11] presented a method to phase and impute long haplotype blocks.
To our knowledge, targeted HaploSeq is the first method to phase the MHC and KIR loci into a single haplotype structure.
We now apply the inverse method to phase data that was acquired from a fixed MTC cell plated on glass bottom dishes.
Finally, we apply our inverse method to phase data acquired from light that was reflected from stress fibers and focal adhesions on the ventral plasma membrane, which lies in apposition to the coverslip-buffer interface.
Methods to phase rare variants are being developed, 5 and as more sequence data becomes available then LD relationships between variants will become better understood, and it may be possible to assign even quite rare variants to known haplotypes.
Use of such databases in combination with methods to phase de novo mutations and haplotypes resulting from recent recombinations could both permit increased haplotype quality and reduce the need for genetic and molecular haplotyping in patients from these populations.
We thus opted to compare our method to phasing-based estimates derived from the application of the BEAGLE phaser [ 18] to our data.
We developed a method to detect phased KIR gene-content haplotypes based on PCR sequencing of amplicons.
Since extension of the above method to a phased 2-SNP haplotype is equivalent to extending the method to a candidate locus with four alleles, we consider those two scenarios together.
In comparison to phasing methods based on statistical inference, LRP has been reported to be 1,000 times faster with a 34% lower error rate than fastPHASE [ 6].
We have extended the SHAPEIT2 method to use phase-informative sequencing reads to improve phasing accuracy.
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