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As an alternative method to pathway analysis, nitrogen assimilation can be investigated through PACE analysis.
The prediction that ribitol-5P is a substrate of EFI-501083, combined with genomic context and kinetic analysis implicates it as the enzyme that catalyzes the orphan phosphatase reaction in the riboflavin biosynthetic pathway sought for over 50 years, illustrating the potential applications of the method to pathway discovery and deeper biology.
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We report also on the results of some experiments applying this method to pathways stored in KEGG, which reveal novel biochemical pathways.
Others have examined multidimensional data sets that include different genome-scale measurements simultaneously in the context of pathways [21], [22], [23].They apply statistical method to measure pathway enrichment and use gene-expression data to assess variation of pathway activity.
An effective method to optimize pathway efficiency may be to use genes from different organisms [ 17].
GSEA was performed here as an independent and complementary method to the pathway analyses using DAVID and MetaCore, which were performed only on the differentially regulated genes.
When applying the correlation method to the pathway model examples, two problems arise regularly that have not been discussed by Jacquez and Greif [ 25].
One method to determine pathway enrichment is with contingency table tests: chi-square test, Fisher's Exact test, or a binomial test [see 9 for a review].
However, because GO semantic similarity scores have been integrated into our approach as one of types of biological evidence, we used a different evaluation method to measure pathway biological function.
The identification of the Wnt pathway, a known BBB inducing signaling pathway, validates this database as a method to identify pathways that may be important for BBB formation.
The identification of the BBB inducing Wnt pathway validates this database as a method to identify pathways that may be important for BBB formation.
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