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Our studies demonstrate that electroporating dsRNA into tick eggs is an efficient, less labor intensive and less traumatic method to newly hatched eggs to target genes exclusively expressing in tick embryos.
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It is also an efficient method to classify newly cloned individual miRNAs into existing miRNA families.
As far as we are aware, this is the first report that compares the GCPSR method to other newly developed tree species estimation methods.
To determine the relative levels of reservoir competence in as many potential host species as possible, we applied this method to sample newly molted nymphal ticks that fed as larvae on a range of potential wildlife hosts.
Furthermore, we apply our method to the newly sequenced genome of Anopheles darlingi where we show that our combined score (cscore) performs well by identifying 44 clear homologs of known pre-miRNAs in Anopheles gambiae amongst the top-scoring candidates.
We monitored translation more globally by immunocytochemistry for the activated, phosphorylated forms of eIF4E and 4EBP, live imaging with a sensorin-based translational reporter (Wang et al., 2009), and the recently described ribopuromylation method to visualize newly synthesized proteins (David et al., 2012).
Using a similar method to identify newly-hired workers, Haefke et al. (2013) report that the share of newly-hireds represents around 8%% of the total number of workers in the United States in an average quarter between 1979 and 2006.
This makes our method applicable to newly emerged viruses in which genome sequences are unknown.
In this paper, we employ the Taguchi method to optimise our newly developed time-modulated chemical vapour deposition (TMCVD) process.
Research on basic cellular processes involving local production or delivery of proteins, such as activity-dependent synaptic modification in neurons, would benefit greatly from a robust, nontoxic method to visualize selectively newly synthesized copies of proteins of interest within cells, tissues, or animals.
The optimal method to validate a newly developed instrument is the comparison with a so-called gold standard, which measures the same construct.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com