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In this chapter, we present a simple and powerful method to either know the DNA specificity of a wild-type protein or design new specificities by using the protein design algorithm FoldX.
It may seem straightforward to use a molecular dating method to either accept or reject the possibility of a recent human influence in the origin of the allopolyploid CD genome.
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We demonstrate methods to either screen or select active Cas9 mutants and use the screening technique to isolate functional Cas9 variants with a heterologous PDZ domain inserted within the protein.
These different strategies will be described, including in vitro approaches to promote the vascular ingrowth from the host tissue, as well as methods to either increase the cell viability in 3D constructs or produce a vascular network prior to its implantation.
All four studies employed quantitative methods to either measure awareness of surveillance, reasons for reporting or to compare actual AEFI reports by health professionals.
To explore clinical outcomes more closely, we examined symptomatic and syndromal remission and relapse (as defined in the methods) to either depression or mania as separate events for participants receiving either olanzapine or CMS as the PT.
However, a reliable method to quantify either mutant is not available, partly because the viral genome has polymorphisms in general and the precore mutations are complex.
Composite coatings electroplating could be a valid and relatively low cost production method to codeposite either micro- or nano-particles of Al in Ni matrix.
Enhanced CT represents a comprehensive imaging method to evaluate either mesenteric vasculature status or small bowel appearance, both of which have to be evaluated for a diagnosis of ischemia before development of intestinal necrosis and infarction.
The rat models associated with implant-related osteomyelitis use a direct inoculation method to represent either contamination of an open wound or a surgical site infection.
We constructed deletion cassettes for use with the λ Red-mediated site-directed mutagenesis method to delete either guaBA, htrA or clyA from wildtype S. Typhi Ty2.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com