A potentially significant second advantage of a reversibly assembling colloidal gel is the ability to passage cells without using a trypsinization process or other biochemical cell detachment method, a required step to detach cells from most substrates including current 2D and 3D matrices.
The water displacement method, which is important to detach connected microorganisms from the sediment matrix and for the subsequent filtration step onto membranes, does not influence the growth of target bacteria (Abia et al. 2015a).
In the past, methods of isolation have used proteolytic enzymes to detach cells; thereafter, traditional methods for cell passaging have used trypsin/EDTA mixtures.
Closely related is another possible objection, namely, that it may not be as easy to detach one's method from one's politics as one might think.
Herein, ZnO NWs were grown onto carbon fabrics through a facile hydrothermal method, and the pull-off force to detach an individual ZnO nanowire from CF was measured using a nano-manipulator inside a scanning electron microscope chamber.
We also tried the method using attached cells, however, they were apt to detach during the incubation and the concentration was much lower than that of suspended cells.
In contrast, all the cells stained from the conventional method were rounded up since it requires one to detach the cells.
These methods use mechanical procedures and detergents and/or EDTA to detach the epithelial layer.
Instead of this method, the AFM cantilever tip, replacing the action of ATP binding, could be used to detach the trailing head by injecting mechanical energy into the head.
It is easy to detach yourself from the person on the table and become absorbed by the details of method and anatomy.
