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In the same study, zona drilling (zona opening) resulted in only 43% of the embryos hatching, although this is in sharp contrast to results obtained by Cohen and Feldberg (1991) who applied the same method to cleavage stage embryos; in their experiments, 92% of zona-drilled embryos hatched fully.
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The new methodology is also combined with the existing constraint-modified approach in the R6 defect assessment method to predict cleavage fracture in shallow, blunt-notched SE(B) specimens with a small degree of conservatism.
An alternative method to improve substrate cleavage by increasing the intrinsic sTEVp catalytic efficiency is likely to be challenging.
Thus, we used the RLM-RACE method to map the cleavage sites in one of the predicted SsCBP s (SsCBP1, TC90826).
Chemical cleavage of mismatch (CCM) is a highly specific and sensitive method to induce mismatch-cleavages [ 33].
The D-P-S linker allowed a simple cleavage method to release pexiganan from the fusion protein due to the deamidation reaction at the D-P site under conditions of low pH and high temperature (Hamada and Swanson 1994).
In this work, we employed the 5'-RLM-RACE method to detect miRNA-guided cleavage of target mRNAs of five Va-miRNAs, where the results showed that five potential target genes for the five Va-miRNAs had specific cleavage sites corresponding to their miRNA complementary sequences.
In this paper, we proposed a simple method to accurately measure the "intracellular cleavage efficiency" of Rz.
H. Liu et al. developed a new method to predict HIV-1 protease cleavage site.
Using the high throughput Proteomic Identification of protease Cleavage Sites (PICS) method to simultaneously profile both the prime and non-prime sides of the cleavage sites of nine human MMPs, we identified more than 4300 cleavages from P6 to P6′ in biologically diverse human peptide libraries.
In this study, we have developed a new biotinylated-sortase self-cleavage purification (BISOP) method to achieve high quality purification of several proteins, including a protein heterocomplex, without any affinity tag.
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