When we tested filtering methods for tier one variants after the enrollment of 91 patients, filtering variants to include only those with a minor allele frequency of less than 1 % that were either categorized as DM in HGMD or predicted to be protein truncating reduced the number of unique variants for review from 198 to 30, while capturing all deleterious variants.
Outside the United States, other countries have proposed modifications to this approach, and some suggest a three-tier method that can be applied to different aquatic exposure scenarios (tier 1: fish screening; tier 2: fish partial life cycle; and tier 3: fish full life cycle) (Hutchinson et al. 2000).
This study presents an example of a systematic and simple first tier method to assess the toxicity of degradation products.
This paper proposes a novel tire instant effective radius (TIER) method for real-time estimation of the CG height for LWVs.
Mr. DiIulio told a news conference here after his speech that this two-tier method of grants for some, and vouchers for others, adhered to existing law.
The application of lower tier methods also assumes that the average transfer rate into dead organic matter (dead wood and litter) is equal to the average transfer out of dead organic matter, so that the net stock change in these pools is zero [15].
For GWAS data, the Plink set-based test generated the largest number of significant pathways among the four methods, regardless of tier one or tier two criterion.
Recently, a consensus document has been published on the clinical suitability of CMA as the first-tier method for the study of cases of intellectual disability or congenital malformations (Miller et al. 2010).
A tiered approach, for example, has been advocated as an assessment strategy in which testing would proceed in a stepwise fashion from general screening using simple behavioral methods and neuropathology (tier 1) to the characterization of effects (tier 2) using more specific testing techniques.
Samples were processed in batches of 96 to increase throughput and a two-tier method applied: (i) An initial PCR-ELISA with a cocktail of HR or LR type-specific probes; (ii) A second PCR-ELISA of all HR-positive samples with genotyping using 14 individual HR HPV probes: HPV 16; 18; 31; 33; 35; 39; 45; 51; 52; 56; 58; 59; 66 and 68.
