For Step 2 of the method, the 3 zones of bins in pattern space (blue, orange, and red) were used to define 3 large ROIs in the brain volume by finding the voxels that contributed to the frequency counts in those zones (shown in Fig. 6).
The antibacterial bioassay was made by agar well diffusion method, by measuring the zone of inhibition against the test microorganisms by using Asghari method with a little modification [ 15].
Antagonistic activity of yeast isolates was evaluated by agar well plate method by measuring the zone of inhibition around the wells as per protocol described by Sibanda et al. (2010) against human intestinal pathogenic bacteria like Escherichia coli 0157 H7, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Salmonella typhi and Salmonella paratyphi.
Moreover, different ground conditions and construction stages will require the application of different empirical methods to calculate the zone of influence of deep excavations.
Good agreement is also found between the radiative flux densities calculated using the two-flux model and the zone method.
The coupled simulation of the reactor and the radiant box by means of the zone method, results in the understanding of the behavior of the furnace.
Then, the antibacterial activities against Aloin, AgNP-stabilized Aloin, and pure AgNP (chemical method) were compared and the zone of inhibition was calculated.
In addition, it can be seen that the dispersion of the sediment method always occurs outside the zone of bar movement.
Due to the well established accuracy of the zone method of analysis, the closeness of the two sets of predictions indicates that the two-flux model should prove extremely useful in assessing the thermal effects of changes in design or operating conditions in process gas heaters, particularly because of its computational simplicity compared with the zone method.
The thermal behaviour of a multipass process gas heater has been investigated using the zone method of analysis, with the aim of estimating the accuracy of previous predictions based on an extended two-flux model of the radiation field.
