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Using this method, the viability of ECs after freezing was 90%.
To validate the method, the viability of our corticostriatal organotypic slice cultures was analyzed using a standardized protocol for PI uptake.
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In plasmid shuffle assays (Materials and methods), the viability of cells containing the smc1-D1164E or SMC1 alleles was indistinguishable.
This novel method improved the viability of MSCs and enhanced their function after infusion by directly inhibiting complement and by recruiting factor H, another potent complement inhibitor in serum, onto the surface of the MSCs.
Hepatocytes were isolated with two-step collagenase perfusion method and the viability of the cells was over 80%.
The data presented demonstrate that the method preserves the viability of the microcolonies it detects, thus enabling generation of pure cultures for microbial identification.
This staining method revealed the viability of Neochloris sp. in textile dyeing industry effluent.
This method determines the viability of the organisms after contact with antimicrobials for a specified time period and allows us to evaluate the effects of the potential antimicrobial on biofilms, which play a significant role in numerous human diseases and contribute to treatment inefficiency [ 41– 41].
The adhesive properties of Aeromonas hydrophila LOCK0968 were studied in water with a low concentration of organic compounds, using luminometric and microscopic methods, and the viability of the adhered bacterial cells was evaluated using the colony forming units method.
We highlight how the debate has led to increased understanding of the importance of phylogenetic methods, of the viability of optimality models as tools for testing adaptive hypotheses, and of whether constraint hypotheses are rivals to adaptive hypotheses or provide the backdrop against which we test hypotheses about the evolutionary process.
We examined the utility of this perfusion method to enhance the viability of the thick organotypic brain slice cultures after 2 days and 5 days in vitro (DIV).
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