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In this method, the staining intensity decreases when the reagents disrupt the MMP, and quantification of the cells is based on the depolarized mitochondria membranes.
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For a direct comparison of the two fixation methods, the staining solution was removed after approximately 24 hours when the most satisfactory staining was obtained using both methods.
This method is identical to the root staining and quantification method used for AMF, excluding the staining step.
This was accomplished by using the multiple label method comparing the staining patterns for MyoD, myogenin, NCAM, dystrophin and laminin.
The weighted histoscore method assesses the staining intensity and the percentage of cells stained with that intensity for the full slide.
The method for staining CGs was based on the procedures reported in our previous study [41].
Root length colonization by AM fungi was calculated using a gridline intersect method after staining the roots with trypan blue (Koske and Gemma [1989]).
The method of staining 8-oxoG in mitochondrial DNA was described previously [5], [22].
In conclusion, we have developed a new immunofluorescence method for staining the frozen tissue sections of colorectal cancer by an EpCAM DNA-based aptamer.
We did not perceive any consistent difference in yield or size of amplified RNAs that could be attributed to the method of staining.
The Golgi-Cox method for staining neurons in the brain was performed according to the protocol described by Ranjan and Mallick (2010).
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