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Ryan et al. have named this method the Multiplex CRISPR (or CRISPRm) system.
In comparison to the tissue culture method, the multiplex assay and WHO-recommended protocol had the same sensitivity (100%) and specificity (98.3%) for FluA, and the same sensitivities and specificities for the H7 (100%) and N9 (100%) genes in the clinical specimen (Table 3).
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The first method uses the multiplex phase cycling to acquire MQMAS spectra from various coherence transfer pathways simultaneously.
TPE spectra of the fluorescence [ 14– 21] from various fluorophores have been measured by various spectroscopic techniques including the wavelength scanning method [ 14– 16], the multiplex method [ 17] and the Fourier-transform (FT) method [ 18– 218.
A detailed description of the methods of the multiplex assay performed at the Core facility has been described previously by others [20].
Primers, probes and amplification methods of the multiplex PCR used in this study are described in reference 4 for 'laboratory 2'laboratory
Regression analysis revealed a linear relationship without intercept (y = 0.714x, R = 0.987, p < 0.001), indicating almost complete agreement between the methods while the multiplex technique systematically underestimated the IL-6 concentration in the samples by about 30%.
First, the sensitivity of the MIA-TF method was higher: the multiplex TFs assay needs at least 500 ng of nuclear extracts for the detection of TF activity according to the instructions, while the MIA-TF method detected the activity of TFs using 50 ng of nuclear extracts.
Developments in the PCR technology and discrimination methods combined with the multiplex assays have improved the detection of coinfections with reduced cost and sample volumes required for analysis [ 40].
All cell lines were tested and authenticated in February 2008 by the Genetic Core Facility at the German Cancer Research Center (Heidelberg, Germany) using the method Multiplex cell Contamination Test (McCT) (http://www.dkfz.de/gpcf/contamination-control.html).html
To test the overall performance of the method, multiplex PCR reactions were carried out for over 700 GAS strains.
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