With the k-means method, the expression levels for each condition (time and infection status) of the SLA/PrV differentially expressed probes set were clustered in three groups.
With this method the expression levels of Kir4.1 and aquaporin-4 were quantified in glutamine synthetase positive Müller cells.
In the MICs method, the expression products of cecropin and moricin were diluted to final concentrations of 20, 10, 5, 2.5, 1.25, 0.625, 0.313 or 0.157 µmoL/L, and added to bacteria at logarithmic phase.
By this method, the expression measure is given in log2 base.
Depending on the method, the expression may be estimated using probe level expression or some computed probe set level expression index.
To evaluate the performance of the method, the expression levels obtained using Northern blotting and RNA array were compared (Fig. 1A,1B,1C,1D and Fig. 2).
Using this more sensitive detection method, the expression of nine genes turned out to be significantly up-regulated in the zur mutant (Table 2).
Using the enhanced chemiluminescence (ECL) method, the expression of each receptor was confirmed using anti-LPA1, anti-LPA2, or anti-LPA3 antibodies (Fig. 1).
The posterior mean of μ i, of gene i is calculated by: In our proposed method, the expression level of gene i is estimated by.
To validate the microarray data by an independent second method the expression levels of 5 genes (Table 1) were assessed by real time PCR.
