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Within each DNA extraction method, the efficiencies of extraction amongst different cell amounts were compared by Kruskal Wallis analysis.
For application of the 2-ΔΔCt method, the efficiencies of all measured gene amplifications were first examined using diluted samples and confirmed to be appropriately equal.
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In this method, the efficiency of DAMAS increases with compression ratio.
The results showed that, when microemulsion flooding is applied, the displacement efficiency is 21.5%, whereas with the conventional method the efficiency is 41%.
In this method, the efficiency is higher compared to our results.
In our method, the efficiency is further improved by injecting TALEN mRNAs fused to the 3′UTR of the Xenopus DEADSouth gene into oocytes, which are then transferred into a host female frog, where they are ovulated and fertilized.
For method 3, the efficiencies in table 4 are rather equal in all scenarios.
The expression levels of the samples were calculated using the ΔCt method, incorporating the efficiencies of each primer pair.
Although remarkable progress has been made in developing novel reprogramming methods, the efficiency and fidelity of reprogramming need to be improved in order increase the experimental and translational utility of reprogrammed cells.
Furthermore, as improvements are made in sequencing-by-synthesis methods, the efficiency and power of the 5'tagged PCR method is predicted to increase.
Moreover, in all methods, the efficiency of reprogramming is very low, suggesting that additional components of the reprogramming pathways remain to be identified.
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