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In this method, the cell is partitioned into two regions.
For the second method, the cell adhesion peptide, RGD, was chemically conjugated to a thiol-active maleimidylated form of the scaffold.
With the same sampling method the cell type composition of the cultures was evaluated at 1 and 7 DIV on 3 coverslips from 3 distinct experiments.
Using a developed gel casting method, the cell sheets can be transferred to a flat surface and will maintain structural organization long-term.
In order to determine the viable cells in proliferation or cytotoxicity assays, the MTS colorimetric method (the Cell Titer 96® Aqueous One Solution Cell Proliferation Assay, Promega, USA) was used.
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After an initial design stage by the finite element method, the cells have been fabricated and characterized.
Comet assay has considerable advantages over the other cytogenetic methods because in this method, the cells do not require to be mitotically dividing.
In the live cell binding method, the cells were incubated with peptides in cell growth medium for two hours at 37°C.
To demonstrate the feasibility of the method, the cell-free supernatant derived from a culture of a mAb2-producing CHO cell line was analyzed for aggregate formation.
In the second method, the cells were captured in microstructured polydimethylsiloxane (PDMS) stamps according to [ 28].
In this method, the cells were first fixed with a paraformaldehyde/glutaraldehyde solution as described earlier.
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