About 250 ng of [ 23]LMW RNA was used as the input for miRNAs labelling using the Array900 miRNA Direct kit (Genisphere Inc., Hatfield, PA, USA), a tagging method that allows direct labeling of mature miRNAs without PCR amplification.
We are aware of another method that uses direct scheduling (Lahoti O, personal communication).
The MCLs developed by Wilson and his colleagues provide a quantitative method that permits direct assessment of drug penetration through solid tissue [17, 18].
At its core is a new method that enables direct intersection and Boolean operations between designed geometry (objects bounded by NURBS and polygonal surfaces) and scanned geometry (objects represented by point cloud data).
Interestingly, this naive co-occurrence-based method performs at par to the SLK method that extracts direct connectivity statements.
Here, we employ a RABV ∆G-based method that allows direct transduction of cell bodies, permitting the tracing and complete 3D reconstruction of dendritic and axonal arbors of sparsely labeled neurons.
We have developed the first directed evolution method that allows the direct selection of GPCRs stable in a chosen detergent from libraries containing over 100 million individual variants.
Future efforts to extend and refine our analyses could theoretically produce methods that could direct an investigator to a set of model species that would be well-suited to studying a particular human disease gene or disease class.
If the reviewers and the editor consider it important, we could include a section in the Methods that would direct compare the two studies, similar to the following: First of all, the backpacks used by Anisimov (2014) weighed 3g, i.e. three times as the ones used in our study, and birds showed a prolonged habituation period.
If it necessary to kill a spider, avoid methods that place you in direct or close contact with the spider, especially if it is of a dangerous species.
