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Figure 8 Effective range of overlap correction method tested by olivine-hosted melt inclusion.
Following the method tested by Hinz et al (2012), we calculated three sum scores: function, symptoms and a total score.
According to the method tested by Pfaffl, the relative expression ratio of a targeted gene was calculated based on efficiency and the Ct compared with a reference gene (18S).
According to the method tested by Tichopad [ 23], the relative expression ratio (RR) of CD105 gene was calculated based on Eff and the Ct comparative with a reference gene (GAPDH) in a sample.
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For the text classification test, the HFA method outperforms all methods tested by an average of 1.5 standard deviation.
For the image classification test, the HFA method outperforms all the methods tested by an average of one standard deviation with respect to classification accuracy.
Moreover, it yields results similar to those of TaqMan Gene Expression Assays, a widely accepted method for validating microarray results, and other more complicated and more expensive quantitative methods tested by the MAQC project.
Cumulative recurrence curves were analyzed using the Kaplan Meier method and tested by Wilcoxon's method.
The distant recurrence rate was analyzed using the Kaplan Meier method and tested by Wilcoxon's method.
The purpose of the present study was to develop a molecular genotyping method test by using a real time PCR hybridization probe and applying it to the analysis of C1843T mutations of the Sus scrofa RYR1 gene.
p values from three independent experiments were then combined using Fisher's method and tested by χ test (see 'Detailed data analysis' in 'Materials and methods' for details).
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