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With a method supplied by the letter writer and a plot by a story that he remembered of an actual serving maid who preserved her virtue and was, ostensibly, rewarded by marriage, he began writing the work in November 1739 and published it as Pamela; or, Virtue Rewarded a year later.
Finally, the automated STL generation method supplied by the software was used at the default "optimal" quality to generate the surface enclosing the segmented voxels.
DLS data were analyzed by using the CONTIN method supplied by the manufacturer.
The relative quantification of the transcripts was analysed using the comparative threshold cycle method supplied by the manufacturer.
Fasting glucose, the primary outcome measure, was measured using a timed end point method supplied by Beckman Coulter for the Synchron LX Analyzer, a method accepted by the Centers for Disease Control and Prevention.
Using the blood and urine samples collected during the clinic visit, total and high-density lipoprotein (HDL) cholesterol were assayed by the cholesterol oxidase method supplied by Boehringer Mannheim Diagnostics on a Roche COBAS Fara analyzer (Indianapolis, IN).
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Using standard methods supplied by the manufacturer, one-dimensional SDS-PAGE was performed.
Acid detergent fibre, neutral detergent fibre (NDF) and crude fibre were determined with an Ankom 2000 fibre analyser (Ankom Technology, NY, USA) as per the methods supplied by the manufacturer.
Alternatively, the transformation steps were skipped and vsn [ 18] or the normalization methods supplied by BeadStudio (average, rankInvariant, cubicSpline) are used for normalization.
qPCR was performed in either a 7900HT, 7500HT or StepOne PCR System (Applied Biosystems) and analyzed by using the ΔΔ-Ct method as supplied by the manufacturer (Applied Biosystems).
qPCR was performed in either a 7900 HT, 7500 HT, or StepOne PCR System (Applied Biosystems) and analyzed by using the ▵▵-Ct method as supplied by the manufacturer (Applied Biosystems).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com