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Exact(5)
In the immuno-enzyme polymer (indirect) method, sections were incubated with dilutions of primary antibodies at 4°C overnight.
For the immunoperoxidase method, sections were incubated with 1 : 50 diluted anti-human TF antibody (4509; American Diagnostica, Stamford, CT, USA) overnight at 4°C.
Using the MM method, sections were labelled by either anti-Pax7 or anti-NCAM for SC identification together with staining for Dlk1, laminin and DAPI.
After staining with the ABC method, sections were visualized with DAB and positive cells were colored brown in the dorsal horn of spinal cord on each side and were counted at 200× 400× magnification.
For the anti-activated caspase-3 method, sections were deparaffinized, rehydrated, and then incubated in 1 mg/mL hyaluronidase (Sigma-Aldrich Corp, St Louis, MO) in PBS for 60 minutes at 37°C and washed in PBS.
Similar(55)
Among pairs of articles in PMC, method sections are found to be the most repetitive (frequency of similar pairs, methods: 0.029, introduction: 0.0076, results: 0.0043).
Gender, age and subsample membership (see method section) were entered as control variables.
Where abstracts were unclear, methods sections were reviewed.
In the first search, the Methods sections were reviewed for indication of use of cultured cells.
In "Method" section is explained the method used for this purpose.
Methods The method section is written well.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com