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To build the SIMCA method, samples were divided randomly in a training and validation set according to the Kennard-Stone algorithm, where 2/3 of the 118 samples were set for calibration and the remaining for validating the model.

To demonstrate the usefulness of this method samples were also analyzed by employing Clarke's HPLC methods (Anthony et al. 2003) for determination of paracetamol, phenacetin and methaqualone where as Tibbels and Smith method (Tibbels & Smith 1988) was used for analysis of saccharin.

In determining the linearity of the method, samples were prepared by spiking blank rat plasma with standard solutions (prepared in methanol) of bergapten to the concentrations: 2, 4, 8, 20, 40, 100 and 100, 200, 500, 1000, 2500 and 5000 ng mL−1 for the calibration curves.

Because this is a subjective method, samples were scored independently by two individuals, with similar results.

With the LC-UV method, samples were previously found to be negative for monensin, salinomycin, and narasin.

Protein concentration was determined by the Bradford method, samples were normalised for protein concentration (25 50  μg), and electrophorised on 10 12.5% SDS PAGE.

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Similar(51)

In this second method, samples are drawn of the carrier phase rather than of the data symbols.

For both methods, samples were scored as "failed amplification" when only one or none of the three replicates amplified.

In this method, sample was centrifuged at 10,000 rev/min for 30 min and the pH was adjusted to 7.6.

For each site and for each sampling method, 500 samples were simulated.

Methods: Blood samples were obtained from consented 12 healthy volunteers.

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