Conventional open methods require up to a 10 cm incision over the trochanter.
The same task performed by the state-of-the-art non-linear registration methods may require up to several days.
All culture-based methods may require up to 48 hours for microbial identification: while waiting for microbiology results a broad spectrum antibiotic therapy has to be started.
The bottom-up method requires a price tag to be placed on each item (eg, on each dose of different medicines, on each consultation, on each diagnostic investigation, on a day and/or night in a hospital bed, on a lost work-day, etc).
In a clinical setting, indeterminate samples by Focus (currently used as a stand-alone method) require follow-up testing at a later date to demonstrate seroconversion.
In contrast to other approaches, setting up this genotyping method requires an affordable investment.
Luckily, baking beetroot in a steamy foil parcel (our favoured method) requires little active cooking or clearing-up time, and produces perfectly tender roots.
This method requires larger number of cells to be labeled (up to several millions).
Again, this method requires the analysis of many markers to end up with only few cervical cancer specific methylation markers.
Future studies are needed to elucidate whether the method requires the introduction of an additional SPE clean-up step, like in HCl/MeOH method case.
