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Although this method requires quite a few computations, it may import error for the analysis of the studied mechanism.
Moreover, the method requires quite pure carbohydrates free from aa residues, peptides, and organic acids, to avoid interference with EC detection.
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The method is based on recursive solution of mixed-integer linear programming (MILP) optimisation model that requires quite moderate number of binary variables.
However, these methods all require quite involved operations on the raw input data prior to or during the statistical testing step, and hence they are not as generally applicable to most situations as the p-value based methods.
Their method requires no travel at all.
This method requires a facilitator.
Although our method requires the manual selection of the PCs used for feature selection, no pre-knowledge about the ranking between classes is needed and how the classes should be ranked is quite clear from the PCs.
Either method requires practice.
This method requires a LOT of patience!
However, this method requires a warning.
This method requires speed and dexterity.
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