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Meanwhile, this method requires expensive heavy ion accelerometer and restricts the fabrication and application of the solid-state nanopore severely.
However, this method requires expensive DNA sequencer and needs to further analyze after obtaining the raw sequences data, which restrict the use of this method more or less.
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Generally, these methods requires expensive instruments, qualified personnel, sample pretreatment and are hard to use in environmental conditions.
Many of the methods require expensive equipment.
Gas-phase methods require expensive and large-scale vacuum equipments.
Since, some of these methods required expensive equipment(s) and/or special treatment.
All of these methods require expensive instruments and sample cleanup procedure.
These methods require expensive instruments, time-consuming sample pre-treatment and skilled persons to operate them.
Generally, these methods require expensive and sophisticated equipment, high temperatures with long processing times, expensive precursors and high consumption of electric energy.
However, these patterning methods require expensive mask, complex multi-step processes, and metal catalysts in some cases, and deposition of seed layer on substrates.
Such method is subjective and requires an experimented examiner while objective methods require expensive material, such as 3D motion system and pressure insoles.
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