An additional method provides single-step isolation of complete phagemid DNA, via a thermostable DNA polymerase and DNA ligase, using an inverse PCR application with 5′-phosphate oligonucleotides [ 20, 21].
The temporal method provided single estimates of N b for the integrated and segregated lines across the sampled generations (Fig. 5; Table S5b).
The chequerboard is an easy to perform, high-throughput method which provides single time-point evidence of bacterial growth inhibition, and generally results in an overestimate of synergistic interactions [ 22].
The numerical algorithm is a generalization of the standard tridiagonal method and provides single-pass solutions which satisfy the original difference equations to within the numerical accuracy of the computer.
This method provides a single transporter solution for effective fermentation on lignocellulosic feedstocks.
Each scoring method provides a single value for each alignment column that enables the evaluation of conservation (positive value) or divergence (negative value) at every column of the MSA.
The proposed method provides a single model of travel on an urban road network, via the identification of an optimal coefficient within a class of distance metrics, known as Minkowski metrics.
In contrast, the hook method provides strictly single-chip estimates which are based on the intensity information of only one particular chip.
This method provides the individual coercive field of single-domain nanostructures.
A number of quantitative proteomic techniques are currently in use, but no single method provides comprehensive proteomic coverage and previous reports have suggested that individual methods preferentially detect proteins with certain biophysical characteristics [26].
