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And then on to the most controversial bit of all: how do you make your tea? Ian Bersten, an expert among us, shared his method, previously published in the International Journal of Tea Science (pdf), he argues that surface area is more important than brewing time; "the finer, smaller the tea leaf the stronger the cup".
Protoplast transformation was conducted using a 96-well plate (U-bottom plate; U96 MicroWell Plates, Nalge Nunc International, NY) based on the method previously published (Kato and Jones [2010]).
The method was identical to the method previously published [10].
Aa Omp16 and Omp29 were purified followingthe method previously published [11].
Batch A based on a denaturing step during purification, applying a method previously published for the purification of Bet v 1 isoforms with minor modifications [12], [13], [14], and batch B prepared from soluble bacterial lysate following an optimized production protocol.
Mitochondrial ROS were detected using a method previously published.
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A comparison was performed against three of the best skull stripping methods previously published: Brain Extraction Tool (BET), Brain Surface Extractor (BSE), and Hybrid Watershed Algorithm (HWA).
Fifty field samples were also used to evaluate the utility of single PCR and duplex PCR assays, and the detection results were compared with those of the PCR methods previously published.
Immunoblotting was carried out according to methods previously published by others [38].
The present work builds on methods previously published concerning both tumor evolution at constant fitness [15] and negative clonal selection in the absence of tumor evolution [17].
The diblock glycopolymers were synthesized according to similar methods previously published.
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