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This method permits detection of the bicistronic and any putative monocistronic transcripts, as shown in previous reports using the same transgenic mouse line as well as in other animal and cellular models [7], [19], [22] [24].
Notably, the ABC method permits detection of gastric cancer in PG-negative subjects, addressing the fact that most diffuse cancer is H. pylori-positive but PG-negative.
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The sensitivity of the method permitted detection of ~3 fmol DA.
The sensitivity of the method permitted detection of ∼3 fmol DA.
This simple method permitted detection, at a glance, of the presence of the substitute fixative, and avoided the use of smelling when in doubt.
The versatility of our method also permits detection of interactions between nuclear and non-nuclear proteins in either the nucleus or the cytoplasm, as well as the interaction between two plasmid-expressed proteins or between a plasmid-expressed protein and an endogenous protein.
Result indicated that the method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h.
The method permits the detection of cytosine methylation at single-base resolution yet at affordable costs.
Sodium carbonate treatment greatly reduced the actin signal and, coupled with SDS-PAGE and the gel gridding method described in the methods section, permitted detection of a total of 468 non-redundant proteins in the intact LB, LME, and LMD fractions (Table S1).
The most widely used method – the chromogenic modification of the limulus amoebocyte assay (LAL) [ 19, 20] – permits detection of endotoxin in picogram quantities and has become the standard assay system used to detect endotoxin contamination in pharmaceutical manufacturing.
Compared with other laboratory diagnostic methods, electron microscopic excels with respect to rapidity and the open view that permits detection and identification of both novel agents and those not considered by the clinician.
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