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Unlike the rotation-advancement method, our technique does not leave a transverse scar at the alar base.
Experimental results indicate that compared with the conventional method, our technique gives a smaller noise residual.
Furthermore, using immune sera raised against native haemoglobin and its fragments, as a differential diagnostic method, our technique is suitable for parallel demonstration of haemoglobin and its fragments; in this way, the origin of the bleeding can be localised (Ottó et al, 1995).
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Compared with the currently employed methods, our technique has the advantages of a good reproducibility, an accurate sterility that can be maintained throughout the isolation procedure and a high yield of pure endothelial cells potentially able to maintain functional properties intrinsic to the endothelium in situ.
We note that most of the existing methods in the literature used to investigate such problems are based on the finite element method, hence our technique is new in tackling integro-differential equations.
Given its benefits over the previous methods, our novel technique could greatly facilitate future work in wild animal genetics.
To validate the docking method, we first applied our technique to the rebuilding of a known TCR/p/MHCII crystallographic structure.
So, comparing with the reported methods of libraries construction, our technique is the most simple and efficiency one, it is convenient to be applied.
As detailed in the Methods, our structure-based technique involves threading of mutant amino acid sequences onto native PR and RT structures obtained from the Protein Data Bank (PDB) [ 26], as well as application of a computational mutagenesis methodology incorporating a four-body, knowledge-based, statistical potential energy function [ 27].
This paper is organized as follows: section 'Method' describes each step of our technique in detail.
Our method and technique is very simple as compared with other methods.
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