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For protein analysis, OCLs were purified following a modification of the method originally reported by Tezuka et al. (19).
Angiogenesis was studied by culturing rings of mouse aorta in three-dimensional collagen gels with some modifications of the method originally reported for the rat aorta (4).
The addition of magnesium chloride was necessary for chromatographic resolution of AcCoA and 3HB-CoA, which co-elute in the method originally reported by Shimazu and co-workers.
Cell numbers were accurately quantified by flow cytometry, based on a single-tube platform with self-made cell-beads as internal controls, a method originally reported by Harrison et al. [ 32] and modified by us [ 33].
Accurate enumeration was carried out by FCM based on a single-tube platform with self-made cell-Beads as internal controls, a method originally reported by Harrison et al. [ 14] and modified by us [ 15].
We employed a method originally reported in (David and Alm 2010) to infer event penalties leading to minimal average difference in genome size between ancestor and descendant across the branches of the species tree (genome flux): where pGS i and cGS i denote ancestral (parent) and descendant (child) genome size for branch i, and 196 is the number of branches in the species tree.
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The ECFCs assay method was that originally reported by Ingram et al [14].
We modified a meta-analysis method that was originally reported by Rhodes et al. [ 7] and implemented it in the R statistical computing environment [ 44].
Interaction analysis was done using multiple methods, including those originally reported and the most powerful methods described.
3 The results of all these methods have been originally reported in [36].
The review also introduced two supervised methods, one of which was originally reported in (Jiang et al., 2007).
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