Sentence examples for method of transcription from inspiring English sources
The almost total lack of introns in the genome and the poly-cistronic method of transcription employed by trypanosomatids greatly facilitate the prediction of open reading frames and thus aid in proteomic and functional genomics studies such as mass spectrometry, RNAi, overexpression and epitope tagging.
Similar method of transcription factor binding sites analysis was used in Iwanaszko et al. [ 15].
Additionally, the transcription factors from the preferred method of transcription factor mapping also contribute to many false regulatory links when used in the regulatory elements integration step.
Many significant mechanical methods of transcription have been devised.
This added directionality (Bezemer & Mavers 2011) and depth to the emotional and qualitative descriptions of movement from layer one, as opposed to other methods of transcription (e.g. Heath et al., 2010a, b) which lets the reader retain more agency by letting them design a course of their own in reading the transcripts.
The restricted developmental and spatial expression of SPAM1 [ 5, 7, 21], as well as the unique transcriptional mechanisms employed during spermatogenesis (reviewed in [ 22]), may render SPAM1 unamenable to traditional methods of transcription and promoter analysis.
Structural homologues of the genes encoding for 70 kDa heat shock proteins were targeted using the standard method of reverse transcription from the total RNA extracted from heat shocked P. apterus adults (1 h after the exposure to +41°C for 1 h).
Our results might also be influenced by the method of reverse transcription.
Finally, it is important to keep in mind that any tampering with RNA isolates (including linear amplification, method of reverse transcription, freeze-thawing, etc).
However, the correlation between the fold changes generated by qRT-PCR, a highly reliable method of measuring transcription [ 20, 22] and those found by Affymetrix and Illumina were outstanding, exceeding a Pearson correlation of 0.976.
Two-step real-time qPCR has the added advantage that cDNA is more stable, but, even with one-step real-time qPCR; transcriptomic profiles are skewed to some degree always in direct accordance with the method of reverse transcription used.
