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The method of substrate binding in Cas5c must be significantly different to that observed in Cas6 proteins, because the active sites of the two families are in different locations.
Since biologically relevant substrates might differ from theoretical activities inferred from in vitro experiments – ' just because it can, does not mean it does' – the best method of substrate discovery is to identify protease-cleaved substrates in complex milieus (López-Otin and Overall, 2002; Tam et al, 2004).
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In addition, new methods of substrate pre-treatment before its loading into the fermenter should be developed in order to prevent the foam formation in biogas digesters.
Such an aim would require systematic methods of substrate finding, which are beginning to become available [39], [6].
We conclude that methods of substrate prediction which evaluate chemical features of amino acids within these key regions may be better able to predict substrate specificity.
In addition, the kinetics of enzyme inhibition by the low concentration Hg2+ has been studied using the kinetic method of the substrate reaction.
The fabrication of micro-resonators, made from porous silica ridge waveguides by using an electrochemical etching method of silicon substrate followed by thermal oxidation and then by a standard photolithography process, is reported.
Strategies in metabolic engineering of E. coli can be classified as four main methods: improvement of substrate or product transportation, enhancement of pathways directly involved in the succinate production, deletion of pathways involved in by-product accumulation, and their combinations.
With respect to the ligand-based method for substrates of OATP1B1, a pharmacophore model was generated with published Km values, and it demonstrated that the key molecular features for substrate transporter interactions appeared to be two hydrogen bond acceptors at either end of a large hydrophobic area.
Unlike previous studies, this method is independent of substrate thickness because it relies only on the surface characteristics of the substrate in the surrounding environment.
Oblique silver nanocolumns grown using OAD method without rotation of substrate have shown surface-enhanced Raman spectra (SERS) [53] and surface-enhanced fluorescence (SEF) [54] which can give useful and desired results in detection of minute amount of chemicals and viruses.
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