Sentence examples for method of splicing from inspiring English sources

The mutants carrying a deletion in the auto-inhibitory domain (from amino acid residue 284 to 454) of IRF7 and the substitution of Ser396 with phosphomimetic Asp located in the carboxyl terminus of IRF3 were generated by the method of splicing by overlap extension and cloning into pcDNA3.1myc-His and designated as IRF7m and IRF3m, respectively.

SAW is not only a standalone method for splicing event identification, but could also be embedded into other splicing event identification method as a short read filter to increase the speed and accuracy of splicing event identification.

Percent spliced in estimates were made using in-house Python scripts implementing previously published methods of splice isoform quantification using exon-exon junction reads (Pervouchine et al., 2013), which avoid the estimation difficulties arising from non-uniform read coverage of exons (Kakaradov et al., 2012).

Identification of splicing events.

This method clusters short reads into splicing fingerprints (MAWs), generates a consensus sequence for each cluster, selects candidate splicing sites, and then aligns consensus sequences to gene models in search of splicing events.

El-Alfy et al. proposed a blind detection method of image splicing using Markov features in both spatial and DCT domains [14].

A method of "slope splicing" is proposed to build a tall-stepped mirror with high precision in a stepped-mirror-based static Fourier transform infrared spectrometer.

This method of fiber splicing is ideal for this application, as it is quick and easy, allowing for a convenient input and removal of the tapering fiber to and from the optical circuit.

A new method of genetic splicing, CRISPR-Cas9, could allow scientists to make designer babies by replacing bits of DNA in the child's reproductive cells.

The IP-10-AT (AT: antagonist) gene with a 6×His tag at the C-terminal was yielded by method of gene splicing to add a methionine after removal of the first 5 N-terminal amino acids.

Total RNA was isolated by the hot phenol method and RT-PCR of spliced and total mRNA of HAC1 and derivatives was performed, as described (Elizalde et al., 2014).