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This view is based on the echidna's method of shuffling around seemingly arbitrarily, and using its snout in a probing manner.
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A library of alpha class glutathione transferases (GSTs), composed of chimeric enzymes derived from human (A1-1, and2 and A3-3), bovine (A1-1) and rat (A2-2 and A3-3) cDNA sequences was constructed by the method of DNA shuffling.
The potential for Chlamydomonas reinhardtii to be utilized for biofuel production was strengthened by developing it for growth in elevated salinity via the selective breeding method of genome shuffling.
Random genome shuffling using selection for growth in the presence of ethanol was carried out for three consecutive rounds of shuffling in three biological replicates (see ' Methods' and Additional file 1: Table S2).
The first selection method encompassed selection for growth in the presence of ethanol after each round of shuffling.
(Cue sound of shuffling papers).
"Of shuffling off," Lotte said.
These results also demonstrate that the different types of shuffled methods are indistinguishable.
These data demonstrate the threshold levels have not affect robustness and the different types of shuffled methods are indistinguishable.
Over 90% of the 1,082 miRNA sequences show a high level of thermodynamic stability at FDR-controlled P-value of < 0.05, based on four types of shuffled methods.
Once registered, shoppers touch a light-sensitive pad and choose their method of payment, without shuffling cash, cards or checks.
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