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Some multicellular glands are tubular and extrude their secretion into a central space or lumen; some, like the oil-producing sebaceous glands of mammals, form their product by complete breakdown of the cells, a method of secretion known as holocrine.
A different method of secretion drainage is the Mucus Slurper, a modified tube equipped with multiple aspirating holes opening on the tip of the ETT.
Norepinephrine, an α-adrenergic agonist, stimulates discharge of the type I granular gland by a holocrine method of secretion with discharge of cellular mRNA [ 24, 25].
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However, while the parasite assembles and routes most surface molecules, such as lipophosphoglycan and GP63, via the classical ER-Golgi-plasma membrane pathway (McConville et al., 2002), it is recognized that many Leishmania-secreted proteins have no N-terminal secretion signal indicating that the parasite employs non-classical methods of secretion (Stegmayer et al., 2005).
Aralar1-overexpressing cells therefore secreted significantly more insulin than control LacZ-transduced cells upon stimulation with 16.7 mmol/l D-glucose, and the magnitude of the increase from Aralar1-overexpressing cells over LacZ-overexpressing cellsol cells was similar between the two methods of reporting secretion.
UCPCR offers a sensitive and specific method of detecting insulin secretion.
The temporary confinement of the volume into which proteins are secreted enhances the sensitivity of the method for low rates of secretion, and makes the measurements more robust across a range of dissociation constants for the capture ligand, compared to open-well configurations.
We found that secretion measured with TIRF-Microscopy is representative of the overall secretion of the cells, thereby validating for the first time the TIRF method as a measure of secretion.
To quantify lung oxidative stress in asthmatic children by measuring concentrations of 8-isoprostane, a marker of oxidative stress, in exhaled breath condensate (EBC), which is a noninvasive method of sampling airway secretions.
The method to increase the efficiency of secretion is to fuse a nucleotide sequence encoding the antigenic protein to a nucleotide sequence encoding the synthetic peptide LEISSTCDA (Guimaraes et al. 2006).
The secretion method of extracellular hsp90α from invasive cancer cells has not been fully elucidated.
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